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Peak area

Detector response from analyte concentration that is directly proportional to the area of the peak. It is a prefer method over measuring of peak height, because peak area is relatively constant even under different mobile phase flow rate and retention time.

Peak area does require well separated peaks, usually resolution of 2.0 or above. When R is around 1.5, it may still be ok to use peak area. When R is less than 1.5, peak height may work better.

Since each detector and system set up is different, the true analyte concentration is then calculated by using a detector response factor (F).

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